ACR X NON ADHERENCE TO ATC CLRNC HAD LTSS FROM CPR Y. PLTDEV.

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AFTER TKOF HDOF ON ACR X ACFT BEGAN TURN BEFORE ENTERING THE SECTOR I WAS WORKING. ACR X HAD EARLIER BEEN GIVEN A 360 FOR SPACING BY PREVIOUS CTLR BUT HAD THEN BEEN CLRED TO FLO TO HOLD. AS PUBLISHED. TRANSFERRING CTLR HAD TO TAKE IMMEDIATE ACTION WITH CPR Y TO STOP HIS CLB BELOW ACR X. CPR Y WENT TO FL207 BEFORE DSNDING BACK TO FL200. WHEN QUESTIONED; ACR X STATED HE WAS AT FLO ENTERING HOLDING. WHEN ADVISED HE WAS ACTUALLY APPROX 50 MI S OF FLO HE SAID HE WOULD CHK THAT. SAID I WAS CORRECT AND HE WAS ENRTE TO FLO. SUPPLEMENTAL INFO FROM ACN 198184. I CLRED ACR X TO FLO TO HOLD. ACR X ACKNOWLEDGED CLRNC. I TRANSFERRED COMS TO NEXT CTLR. CPR Y WAS CLBING TO FL230 DIRECT CAE. ACR X BEGAN R HAND TURN INTO CPR Y. ACR X AT FL210. I IMMEDIATELY AMENDED CPR Y ALT TO FL200 BUT HE HAD CLBED THROUGH IT. CPR Y ACKNOWLEDGED CLRNC AND DSNDED. CPR Y VERIFIED TFC (ACR X) IN SIGHT. SEPARATION LOSS 300 FT AND 2.8 NM. ACR X STATED HE WAS IN HOLDING TURN AT FLO. SUPPLEMENTAL INFO FROM ACN 198182. INBOUND TO FLO VOR WE WERE TOLD TO EXECUTE A 360 DEG TURN FOR SPACING INTO CHARLOTTE. WHILE STILL IN TURN WE WERE GIVEN INSTRUCTIONS TO HOLD AT FLO AS PUBLISHED. WHILE WE WERE PROGRAMMING THE HOLD IN THE COMPUTER; THE ACFT CONTINUED IN THE 360 DEG SHORT OF FLO AND WE CONTINUED AROUND BEFORE LNDG DIRECT TO FLO. I WOULD RECOMMEND WHEN CENTER SPECIFIES PROCEEDING TO A HOLDING FIX; THEY ADVISE THE DISTANCE TO THE FIX AT SUCH TIME SO THAT THE CREW CAN QUICKLY DETERMINE HOW MUCH TIME THERE IS TO SET UP COMPUTER. SUPPLEMENTAL INFO FROM ACN 198183. AS I BEGAN TO PROGRAM THE INSTRUCTION INTO THE COMPUTER THE ACFT ENTERED A SLOW TURN BACK TO THE R FOR ANOTHER 360 DEG TURN. ATC THEN ADVISED US THAT WE WERE SHORT OF FLO; WHERE OUR HOLD WAS TO TAKE PLACE. A CONTRIBUTING FACTOR TO THIS ERROR WAS THE 360 DEG TURN THAT WE GIVEN; COUPLED WITH NEW HOLDING INSTRUCTION.